pGST2-CeEP55
(Plasmid #21501)

• Depositing Lab: James Hurley
• Publication: Lee et al Science. 2008 Oct 24. 322(5901):576-80.

Backbone

• Vector backbone: pGST2
• Backbone size w/o insert (bp): 5000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CEP55-EABR (ESCRT and ALIX Binding Region) domain
• Species: H. sapiens (human)
• Insert Size (bp): 918
• Mutation: Q230 (CAG changed to TAA), Q218 (CAA changed to TAA)
• Entrez Gene: CEP55 (a.k.a. C10orf3, CT111, MARCH, URCC6)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: GTA TAT AGC ATG GCC TTT GCA GGG CTG G

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

PNC to MGS mut in beginning of protein; author says that this is the plasmid that was used in publication.