pB-T-PAF-Clu TL2
(Plasmid
#215382)
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PurposeHigh expression of the Clusterin(Y204S,Y206S,L207S,F209S,L211S,F218S,F219S,F220S) mutant for purification.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 215382 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepB-T-PAF
- Backbone size w/o insert (bp) 7244
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCLU
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SpeciesH. sapiens (human)
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Mutationp.Tyr204Ser, p.Tyr206Ser, p.Leu207Ser, p.Phe209Ser, p.Leu211Ser, p.Phe218Ser, p.Phe219Ser, p.Phe220Ser
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Entrez GeneCLU (a.k.a. AAG4, APO-J, APOJ, CLI, CLU1, CLU2, KUB1, NA1/NA2, SGP-2, SGP2, SP-40, TRPM-2, TRPM2)
- Promoter tetO
Cloning Information
- Cloning method Gene Synthesis
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pB-T-PAF-Clu TL2 was a gift from Franz-Ulrich Hartl (Addgene plasmid # 215382 ; http://n2t.net/addgene:215382 ; RRID:Addgene_215382)