pB-T-PAF-VLDLR_ecto_short isoform
(Plasmid #215395)

• Purpose: Expression of the ectodomain(1-797) of the short isoform of the Very Low-Density Lipoprotein Receptor (VLDLR, UniProtKB; P98155-2) fused to a C-tag (EPEA) in th C- terminal for purification.
• Depositing Lab: Franz-Ulrich Hartl
• Publication: Hartl Lab plasmids (unpublished)

Backbone

• Vector backbone: pB-T-PAF
• Backbone size w/o insert (bp): 7244
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: VLDLR
• Species: H. sapiens (human)
• Mutation: p.Ala798_Ala873del
• Entrez Gene: VLDLR (a.k.a. CAMRQ1, CARMQ1, CHRMQ1, VLDL-R, VLDLRCH)
• Promoter: tetO
• Tag / Fusion Protein:
  • C-tag (EPEA) (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Supplemental Documents:
  • pB-T-PAF-VLDLR_ecto_short_isoform.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry