Skip to main content
Addgene

E. coli Lig-C-HiBiT
(Plasmid #215438)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 215438 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pET21a (+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5409
  • Total vector size (bp) 7515
  • Modifications to backbone
    None known of
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NAD-dependent DNA ligase LigA [Escherichia coli], C-HiBiT, C-10XHis.Thrombin
  • Alt name
    E. coli LigA-C-HiBiT
  • Alt name
    E. coli Lig-C-HiBiT
  • Species
    E. coli
  • Insert Size (bp)
    2106
  • Mutation
    Codon Optimized for bacterial expression, T7 gene10 leader peptide for expression
  • GenBank ID
    WP_094319130
  • Promoter T7 Promotor
  • Tags / Fusion Proteins
    • HiBiT Tag (C terminal on insert)
    • 10xHis-tag with thrombin site (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (unknown if destroyed)
  • 3′ cloning site Xho1 (unknown if destroyed)
  • 5′ sequencing primer T7 promotor primer #69348-3
  • 3′ sequencing primer T7 terminator primer #69337-3
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    was synthesized for us by a commercial source (Genscript).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    E. coli Lig-C-HiBiT was a gift from James Inglese (Addgene plasmid # 215438 ; http://n2t.net/addgene:215438 ; RRID:Addgene_215438)

  • For your References section:

    A general assay platform to study protein pharmacology using ligand-dependent structural dynamics. Ciulla DA, Dranchak PK, Aitha M, van Neer RHP, Shah D, Tharakan R, Wilson KM, Wang Y, Braisted JC, Inglese J. Nat Commun. 2025 May 10;16(1):4342. doi: 10.1038/s41467-025-59658-6. 10.1038/s41467-025-59658-6 PubMed 40346061
Related items: