pDEST-Dok1-V2
(Plasmid
#215452)
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PurposeExpression vector with Dok1 tagged with the BiFC V2 fragment
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 215452 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepDEST-ORF-V2
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Backbone manufacturerhttps://www.addgene.org/73638/
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDOK1
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SpeciesH. sapiens (human)
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Insert Size (bp)1443
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GenBank IDNM_001381.5
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Entrez GeneDOK1 (a.k.a. P62DOK, pp62)
- Promoter CMV
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Tag
/ Fusion Protein
- Venus fragment 2 (V2) (C terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV_F
- 3′ sequencing primer BGH_R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDEST-Dok1-V2 was a gift from Johanna Ivaska (Addgene plasmid # 215452 ; http://n2t.net/addgene:215452 ; RRID:Addgene_215452) -
For your References section:
Dynamic regulation of integrin beta1 phosphorylation supports invasion of breast cancer cells. Conway JRW, Joshi O, Kaivola J, Follain G, Gounis M, Kuhl D, Ivaska J. Nat Cell Biol. 2025 May 26. doi: 10.1038/s41556-025-01663-4. 10.1038/s41556-025-01663-4 PubMed 40419795