pENTR221-VPS35-mScarlet
(Plasmid
#215472)
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PurposeGateway entry vector with VPS35 tagged with mScarlet
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 215472 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepDONR221
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Backbone manufacturerThermo Fisher Scientific Inc.
- Total vector size (bp) 5690
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Vector typeGateway entry vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameVPS35
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Alt namevacuolar protein sorting 35
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SpeciesH. sapiens (human)
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Insert Size (bp)3093
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GenBank IDAF186382.1
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Entrez GeneVPS35 (a.k.a. MEM3, PARK17)
- Promoter none
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Tag
/ Fusion Protein
- mScarlet (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer attL1_F
- 3′ sequencing primer m13_R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pENTR221-VPS35-mScarlet was a gift from Johanna Ivaska (Addgene plasmid # 215472 ; http://n2t.net/addgene:215472 ; RRID:Addgene_215472) -
For your References section:
Dynamic regulation of integrin beta1 phosphorylation supports invasion of breast cancer cells. Conway JRW, Joshi O, Kaivola J, Follain G, Gounis M, Kuhl D, Ivaska J. Nat Cell Biol. 2025 May 26. doi: 10.1038/s41556-025-01663-4. 10.1038/s41556-025-01663-4 PubMed 40419795