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pEF.DEST51-VPS35-mScarlet
(Plasmid #215474)

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Item Catalog # Description Quantity Price (USD)
Plasmid 215474 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pEF-DEST51
  • Backbone manufacturer
    Thermo Fisher Scientific Inc.
  • Total vector size (bp) 8954
  • Vector type
    Mammalian Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VPS35
  • Alt name
    vacuolar protein sorting 35
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3093
  • GenBank ID
    AF186382.1
  • Entrez Gene
    VPS35 (a.k.a. MEM3, PARK17)
  • Promoter EF1a
  • Tag / Fusion Protein
    • mScarlet (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF.DEST51-VPS35-mScarlet was a gift from Johanna Ivaska (Addgene plasmid # 215474 ; http://n2t.net/addgene:215474 ; RRID:Addgene_215474)

  • For your References section:

    Dynamic regulation of integrin beta1 phosphorylation supports invasion of breast cancer cells. Conway JRW, Joshi O, Kaivola J, Follain G, Gounis M, Kuhl D, Ivaska J. Nat Cell Biol. 2025 May 26. doi: 10.1038/s41556-025-01663-4. 10.1038/s41556-025-01663-4 PubMed 40419795
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