pEF.DEST51-ANXA6-mScarlet
(Plasmid
#215475)
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PurposeExpression vector with Annexin A6 tagged with mScarlet
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 215475 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepEF-DEST51
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Backbone manufacturerThermo Fisher Scientific Inc.
- Backbone size w/o insert (bp) 7464
- Total vector size (bp) 8585
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Vector typeMammalian Expression
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameANXA6
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Alt nameAnnexin A6
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SpeciesH. sapiens (human)
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Insert Size (bp)2724
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GenBank IDNM_001155.5
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Entrez GeneANXA6 (a.k.a. ANX6, CBP68, CPB-II, p68, p70)
- Promoter EF1a
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Tag
/ Fusion Protein
- mScarlet (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer EF1a_F
- 3′ sequencing primer BGH_R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEF.DEST51-ANXA6-mScarlet was a gift from Johanna Ivaska (Addgene plasmid # 215475 ; http://n2t.net/addgene:215475 ; RRID:Addgene_215475) -
For your References section:
Dynamic regulation of integrin beta1 phosphorylation supports invasion of breast cancer cells. Conway JRW, Joshi O, Kaivola J, Follain G, Gounis M, Kuhl D, Ivaska J. Nat Cell Biol. 2025 May 26. doi: 10.1038/s41556-025-01663-4. 10.1038/s41556-025-01663-4 PubMed 40419795