pINDUCER20-PTPN12
(Plasmid
#215517)
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PurposeDox-inducible lentiviral transfer vector with PTP-PEST
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 215517 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepINDUCER20
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Backbone manufacturerAddgene plasmid #44012
- Total vector size (bp) 12561
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Vector typeLentiviral
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePTPN12
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Alt namePTP-PEST
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SpeciesH. sapiens (human)
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Insert Size (bp)2331
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GenBank IDNM_002835.4
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Entrez GenePTPN12 (a.k.a. PTP-PEST, PTPG1)
- Promoter mCMV, Doxycycline-inducible
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer mCMV_F
- 3′ sequencing primer WPRE_R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pINDUCER20-PTPN12 was a gift from Johanna Ivaska (Addgene plasmid # 215517 ; http://n2t.net/addgene:215517 ; RRID:Addgene_215517) -
For your References section:
Dynamic regulation of integrin beta1 phosphorylation supports invasion of breast cancer cells. Conway JRW, Joshi O, Kaivola J, Follain G, Gounis M, Kuhl D, Ivaska J. Nat Cell Biol. 2025 May 26. doi: 10.1038/s41556-025-01663-4. 10.1038/s41556-025-01663-4 PubMed 40419795