pET-30a-METTL17-8XHIS
(Plasmid
#215705)
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PurposeExpress METTL17-8XHIS in C41(DE3) cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 215705 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepET-30a(+)
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET-30a-METTL17-8XHIS was a gift from Vamsi Mootha (Addgene plasmid # 215705 ; http://n2t.net/addgene:215705 ; RRID:Addgene_215705) -
For your References section:
METTL17 is an Fe-S cluster checkpoint for mitochondrial translation. Ast T, Itoh Y, Sadre S, McCoy JG, Namkoong G, Wengrod JC, Chicherin I, Joshi PR, Kamenski P, Suess DLM, Amunts A, Mootha VK. Mol Cell. 2024 Jan 18;84(2):359-374.e8. doi: 10.1016/j.molcel.2023.12.016. Epub 2024 Jan 9. 10.1016/j.molcel.2023.12.016 PubMed 38199006