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pRSET-IRES-EGFP
(Plasmid #216151)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216151 Standard format: Plasmid sent in bacteria as agar stab 1 $89

Backbone

  • Vector backbone
    pRSET-B
  • Backbone manufacturer
    Invitrogen
  • Total vector size (bp) 4239
  • Vector type
    Synthetic Biology ; mRNA preparation

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    EMCV IRES
  • Alt name
    Encephalomyocarditis virus internal ribosome entry site
  • Alt name
    Cardiovirus A internal ribosome entry site
  • Species
    Synthetic
  • Insert Size (bp)
    576

Gene/Insert 2

  • Gene/Insert name
    EGFP
  • Alt name
    Enhanced green fluorescent protein
  • Species
    Synthetic
  • Insert Size (bp)
    717

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Kalina Hristova (pRSET-B mCherry, Addgene plasmid #108857 RRID: Addgene_108857). Elizabeth J. Robertson (pCAG-EOMES-EGFP-IRES-Puro).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSET-IRES-EGFP was a gift from Tom Brown (Addgene plasmid # 216151)

  • For your References section:

    Enhancing cap-independent translation of mRNA via in vitro transcription priming. Golojuch S, Largey B, El-Sagheer AH, Brown T. Nature Communications volume 16, Article number: 9205 (2025) 10.1038/s41467-025-64257-6
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