pMetR
(Plasmid
#216329)
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PurposeT7 promoter driving expression of metR
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 216329 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepUC18
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMetR
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SpeciesEscherichia coli
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMetR was a gift from Mark Styczynski (Addgene plasmid # 216329 ; http://n2t.net/addgene:216329 ; RRID:Addgene_216329) -
For your References section:
A Cell-Free Biosensor for Assessment of Hyperhomocysteinemia. Piorino F, Johnson S, Styczynski MP. ACS Synth Biol. 2023 Aug 18;12(8):2487-2492. doi: 10.1021/acssynbio.3c00103. Epub 2023 Jul 17. 10.1021/acssynbio.3c00103 PubMed 37459448