pStx2Trigger
(Plasmid
#216332)
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PurposeT7 promoter driving expression of an Stx2 RNA trigger sequence to activate Stx2 toehold switch
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 216332 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 * | |
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Backbone
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Vector backbonepUC18
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameStx2 RNA Trigger
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SpeciesSynthetic
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pStx2Trigger was a gift from Mark Styczynski (Addgene plasmid # 216332 ; http://n2t.net/addgene:216332 ; RRID:Addgene_216332) -
For your References section:
Protocell arrays for simultaneous detection of diverse analytes. Zhang Y, Kojima T, Kim GA, McNerney MP, Takayama S, Styczynski MP. Nat Commun. 2021 Sep 29;12(1):5724. doi: 10.1038/s41467-021-25989-3. 10.1038/s41467-021-25989-3 PubMed 34588445