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Addgene

pT7RNAPNev-NB1
(Plasmid #216336)

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Item Catalog # Description Quantity Price (USD)
Plasmid 216336 Standard format: Plasmid sent in bacteria as agar stab 1 $89 *
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Backbone

  • Vector backbone
    pUC18
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    T7RNAPN-NB1 fusion
  • Species
    Synthetic

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT7RNAPNev-NB1 was a gift from Mark Styczynski (Addgene plasmid # 216336 ; http://n2t.net/addgene:216336 ; RRID:Addgene_216336)

  • For your References section:

    A modular cell-free protein biosensor platform using split T7 RNA polymerase. McSweeney MA, Patterson AT, Loeffler K, Cuellar Lelo de Larrea R, McNerney MP, Kane RS, Styczynski MP. Sci Adv. 2025 Feb 21;11(8):eado6280. doi: 10.1126/sciadv.ado6280. Epub 2025 Feb 21. 10.1126/sciadv.ado6280 PubMed 39982986
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