pT7RNAPNev-FSR22
(Plasmid
#216338)
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PurposeArabinose-inducible PBad promoter driving expression of the evolved N-terminal portion (residues 1-179) of T7RNAP translationally fused to anti-SARS-CoV-2 RBD DARPin FSR22
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Depositing Lab
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Publication
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Sequence Information
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Sequences (1) — Accept Affinity Reagent Sequence Policy
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Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 216338 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepUC18
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameT7RNAPN-FSR22 fusion
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SpeciesSynthetic
Cloning Information
- Cloning method Gibson Cloning
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pT7RNAPNev-FSR22 was a gift from Mark Styczynski (Addgene plasmid # 216338 ; http://n2t.net/addgene:216338 ; RRID:Addgene_216338)
