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pAAV-CMVenh synapsin-intron-mCherry-1x miR30 SCR
(Plasmid #216393)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216393 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV
  • Backbone manufacturer
    Wilson
  • Backbone size w/o insert (bp) 5154
  • Total vector size (bp) 5865
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mcherry and miR control
  • Species
    Synthetic
  • Insert Size (bp)
    845
  • Promoter CMVenh synapsin

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer GGTTACAAGACAGGTTTAAGGAG
  • 3′ sequencing primer caacgggccacaactcctc
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-CMVenh synapsin-intron-mCherry-1x miR30 SCR was a gift from Veerle Baekelandt (Addgene plasmid # 216393 ; http://n2t.net/addgene:216393 ; RRID:Addgene_216393)

  • For your References section:

    Loss of the lysosomal lipid flippase ATP10B leads to progressive dopaminergic neurodegeneration and parkinsonian motor deficits. Sanchiz-Calvo M, Coccia E, Cawthorne C, Morrone Parfitt G, Torre-Muruzabal T, Tsafaras G, Van Laere K, Cabezudo D, Cascalho A, Van den Haute C, Vangheluwe P, Blanchard J, Bentea E, Baekelandt V. Acta Neuropathol. 2025 Jul 17;150(1):5. doi: 10.1007/s00401-025-02908-0. 10.1007/s00401-025-02908-0 PubMed 40676227
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