pPbHiT_3xmyc_hsp70UTR_hdhfr/yfcu
(Plasmid
#216421)
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Purpose(Empty Backbone) Empty backbone to express gene specific gRNA and carry the homology repair template for CRISPR editing of Plasmodium berghei using the PbHiT system.
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 216421 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepYCs
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Backbone manufacturerQian (2018) 10.1016/j.molbiopara.2018.04.003
- Backbone size (bp) 6000
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Modifications to backboneThe Plasmodium yoelii U6 promoter was changed for the Plasmodium berghei promoter to express the gRNA. A 3xmyc tag followed by the hsp70 3'UTR was added after the synthetic insert cloning site.
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Vector typeBacterial Expression, CRISPR ; Expression in Plasmodium berghei
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Selectable markersPyrimethamine (positive) and 5-fluorocytosine (negative)
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Tag
/ Fusion Protein
- 3xmyc (C terminal on insert)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPbHiT_3xmyc_hsp70UTR_hdhfr/yfcu was a gift from Ellen Bushell (Addgene plasmid # 216421 ; http://n2t.net/addgene:216421 ; RRID:Addgene_216421) -
For your References section:
A scalable CRISPR-Cas9 gene editing system facilitates CRISPR screens in the malaria parasite Plasmodium berghei. Jonsdottir TK, Paoletta MS, Ishizaki T, Hernandez S, Ivanova M, Curbelo AH, Saiki PA, Selinger M, Das D, Henriksson J, Bushell ESC. bioRxiv 2024.04.20.590404 10.1101/2024.04.20.590404
Map uploaded by the depositor.
