A scalable CRISPR-Cas9 gene editing system facilitates CRISPR screens in the malaria parasite Plasmodium berghei
Jonsdottir TK, Paoletta MS, Ishizaki T, Hernandez S, Ivanova M, Curbelo AH, Saiki PA, Selinger M, Das D, Henriksson J, Bushell ESC
bioRxiv 2024.04.20.590404 Article
Jonsdottir TK, Paoletta MS, Ishizaki T, Hernandez S, Ivanova M, Curbelo AH, Saiki PA, Selinger M, Das D, Henriksson J, Bushell ESC
bioRxiv 2024.04.20.590404 Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 216421 | pPbHiT_3xmyc_hsp70UTR_hdhfr/yfcu | Empty backbone to express gene specific gRNA and carry the homology repair template for CRISPR editing of Plasmodium berghei using the PbHiT system. |
| 216422 | pPbU6_hdhfr/yfcu | Empty backbone to express gene specific gRNA from the Plasmodium berghei U6 promoter for traditional CRISPR editing. |
| 216423 | pPbU6_hdhfr/yfcu_Cas9 | Empty backbone to express gene specific gRNA from the Plasmodium berghei U6 promoter and the Cas9 nuclease for traditional CRISPR editing. |