pPbU6_hdhfr/yfcu_Cas9
(Plasmid
#216423)
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Purpose(Empty Backbone) Empty backbone to express gene specific gRNA from the Plasmodium berghei U6 promoter and the Cas9 nuclease for traditional CRISPR editing.
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 216423 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepYCm
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Backbone manufacturerZhang (2017) 10.1016/j.molbiopara.2016.12.010
- Backbone size (bp) 10000
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Modifications to backboneThe Plasmodium yoelii U6 promoter that guides gRNA expression was replaced with the Plasmodium berghei homolog
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Vector typeBacterial Expression, CRISPR ; Expression in Plasmodium berghei
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Selectable markersPyrimethamine (positive selection) and 5-fluorocytosine (negative selection)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPbU6_hdhfr/yfcu_Cas9 was a gift from Ellen Bushell (Addgene plasmid # 216423 ; http://n2t.net/addgene:216423 ; RRID:Addgene_216423) -
For your References section:
A scalable CRISPR-Cas9 gene editing system facilitates CRISPR screens in the malaria parasite Plasmodium berghei. Jonsdottir TK, Paoletta MS, Ishizaki T, Hernandez S, Ivanova M, Curbelo AH, Saiki PA, Selinger M, Das D, Henriksson J, Bushell ESC. bioRxiv 2024.04.20.590404 10.1101/2024.04.20.590404
Map uploaded by the depositor.
