LV-pCMV-FLPo-pGK-mCl-GP33/66
(Plasmid #216481)

• Purpose: Expresses FLPo and mClover3 fluorescent protein with LMCV GP33-43 and GP66-77 epitopes embedded in loop of mclover to initiate immunogenic tumor cells in KPFrt mice
• Depositing Lab: Nikhil Joshi
• Publication: Cheung et al Cancer Cell (2025), doi: 10.1016/j.ccell.2025.09.005

Backbone

• Vector backbone: pLV
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: FLPo Recombinase
• Alt name: FLPo
• Promoter: pCMV

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GATCAGTGTGAGGGAGTGTAAAGCTGGTTTCGATGTACGGGCCAGATATAC
• 3′ sequencing primer: AGGCCTCGGGATTCCTAGGAACAGCGGTTTTCAGATCCGCCTGTTGATGTA

Gene/Insert 2

• Gene/Insert name: mClover3-GP33/66
• Alt name: mCl-GP33/66
• Promoter: pGK

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: AAACCGCTGTTCCTAGGAATCCCGAGGCCTATTCTACCGGGTAGGGGAGGC
• 3′ sequencing primer: AGAGTAATTCAACCCCAAACAACAACGTTTTCAGATCCGCCTGTTGATGTA

Resource Information

• Supplemental Documents:
  • Cheung_t7w_1_LV-FLPo-mCl33-66_pLann.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry