pLox-LV-pCMV-FLPo-pGK-mCl-GP33/66
(Plasmid
#216482)
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PurposeExpresses a lox-p flanked FLPo and mClover3 fluorescent protein with LMCV GP33-43 and GP66-77 epitopes embedded in loop of mclover for initiating and removing immunogenic tumor cells in KPFrt mice
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 216482 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepLox-LV
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Backbone manufacturerDidier Trono
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nameFLPo Recombinase
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Alt nameFLPo
- Promoter pCMV
Cloning Information for Gene/Insert 1
- Cloning method Gibson Cloning
- 5′ sequencing primer GATCAGTGTGAGGGAGTGTAAAGCTGGTTTCGATGTACGGGCCAGATATAC
- 3′ sequencing primer AGGCCTCGGGATTCCTAGGAACAGCGGTTTTCAGATCCGCCTGTTGATGTA (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert namemClover3-GP33/66
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Alt namemCl-GP33/66
- Promoter pGK
Cloning Information for Gene/Insert 2
- Cloning method Gibson Cloning
- 5′ sequencing primer AAACCGCTGTTCCTAGGAATCCCGAGGCCTATTCTACCGGGTAGGGGAGGC
- 3′ sequencing primer AGAGTAATTCAACCCCAAACAACAACGTTTTTACTTGTACAGCTCGTCCAT (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLox-LV-pCMV-FLPo-pGK-mCl-GP33/66 was a gift from Nikhil Joshi (Addgene plasmid # 216482 ; http://n2t.net/addgene:216482 ; RRID:Addgene_216482) -
For your References section:
Distinct T cell functions enable efficient immunoediting and prevent tumor emergence of developing sarcomas. Cheung JF, Hunt BG, Wahed S, Cheng E, Connolly KA, Venkatesan S, Loza JL, Bansal I, Fagerberg E, Kessler EA, Riffard C, Buck J, Attanasio J, Borr ER, Wei W, William I, Fitzgerald B, Joshi NS. Cancer Cell (2025), doi: 10.1016/j.ccell.2025.09.005 10.1016/j.ccell.2025.09.005
