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pLox-LV-pCMV-FLPo-pGK-mCl-GP33/66
(Plasmid #216482)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216482 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pLox-LV
  • Backbone manufacturer
    Didier Trono
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    FLPo Recombinase
  • Alt name
    FLPo
  • Promoter pCMV

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GATCAGTGTGAGGGAGTGTAAAGCTGGTTTCGATGTACGGGCCAGATATAC
  • 3′ sequencing primer AGGCCTCGGGATTCCTAGGAACAGCGGTTTTCAGATCCGCCTGTTGATGTA
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    mClover3-GP33/66
  • Alt name
    mCl-GP33/66
  • Promoter pGK

Cloning Information for Gene/Insert 2

  • Cloning method Gibson Cloning
  • 5′ sequencing primer AAACCGCTGTTCCTAGGAATCCCGAGGCCTATTCTACCGGGTAGGGGAGGC
  • 3′ sequencing primer AGAGTAATTCAACCCCAAACAACAACGTTTTTACTTGTACAGCTCGTCCAT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLox-LV-pCMV-FLPo-pGK-mCl-GP33/66 was a gift from Nikhil Joshi (Addgene plasmid # 216482 ; http://n2t.net/addgene:216482 ; RRID:Addgene_216482)

  • For your References section:

    Distinct T cell functions enable efficient immunoediting and prevent tumor emergence of developing sarcomas. Cheung JF, Hunt BG, Wahed S, Cheng E, Connolly KA, Venkatesan S, Loza JL, Bansal I, Fagerberg E, Kessler EA, Riffard C, Buck J, Attanasio J, Borr ER, Wei W, William I, Fitzgerald B, Joshi NS. Cancer Cell (2025), doi: 10.1016/j.ccell.2025.09.005 10.1016/j.ccell.2025.09.005
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