pIS1 DICER1 long-mut-let-7
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21651||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerAvailable from Addgene (#12179)
- Backbone size w/o insert (bp) 4085
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameDicer 1
SpeciesH. sapiens (human)
Insert Size (bp)4200
MutationMutant let-7 microRNA binding sites
Entrez GeneDICER1 (a.k.a. DCR1, Dicer, Dicer1e, GLOW, HERNA, K12H4.8-LIKE, MNG1, RMSE2)
/ Fusion Protein
- Luciferase (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site Xba I (not destroyed)
- 3′ cloning site Not I (not destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer EBV Reverse (Common Sequencing Primers)
Long means that all functional alternative polyadenylation signals have been deleted.
Mut means that all microRNA binding sites for an indicated microRNA have been mutated.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pIS1 DICER1 long-mut-let-7 was a gift from David Bartel (Addgene plasmid # 21651 ; http://n2t.net/addgene:21651 ; RRID:Addgene_21651)
For your References section:Widespread shortening of 3'UTRs by alternative cleavage and polyadenylation activates oncogenes in cancer cells. Mayr C, Bartel DP. Cell. 2009 Aug 21. 138(4):673-84. 10.1016/j.cell.2009.06.016 PubMed 19703394