pAAVS1-NDi-CRISPRd (ABI-dCas9-mCherry)
(Plasmid
#216531)
-
PurposeTargeting vector for CRISPRd generation
-
Depositing Lab
-
Depositing OrganizationCincinnati Children's Hospital Medical Center
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 216531 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 216531-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 216531-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
-
Vector backbonepAAVS1-NDi-CRISPRi (Gen1)
-
Backbone manufacturerBruce Conklin (Addgene plasmid # 73497)
- Total vector size (bp) 14519
-
Modifications to backboneKRAB-dCas9-mCherry cassette removed (cut w/ Afill and Agel)
-
Vector typeMammalian Expression, CRISPR
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature30°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameABI-dCas9-mCherry
-
Insert Size (bp)5952
- Promoter TRE3G
-
Tag
/ Fusion Protein
- mCherry
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Afill (not destroyed)
- 3′ cloning site Age1 (not destroyed)
- 5′ sequencing primer N/A
- (Common Sequencing Primers)
Resource Information
-
A portion of this plasmid was derived from a plasmid made byABI-dCas9-mCherry insert comes from Stanley Qi (Addgene plasmid # 121513: pHR-PGK-ABI-dCas9-P2A-mcherry)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 216531-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 216531-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pAAVS1-NDi-CRISPRd (ABI-dCas9-mCherry) was a gift from Makiko Iwafuchi (Addgene plasmid # 216531 ; http://n2t.net/addgene:216531 ; RRID:Addgene_216531) -
For your References section:
Pioneer and PRDM transcription factors coordinate bivalent epigenetic states to safeguard cell fate. Matsui S, Granitto M, Buckley M, Ludwig K, Koigi S, Shiley J, Zacharias WJ, Mayhew CN, Lim HW, Iwafuchi M. Mol Cell. 2024 Jan 4:S1097-2765(23)01026-2. doi: 10.1016/j.molcel.2023.12.007. 10.1016/j.molcel.2023.12.007 PubMed 38211589
Map uploaded by the depositor.
