pET-hisTdT
(Plasmid
#216674)
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PurposeExpresses his-tagged N-terminus truncated TdT in E. coli hosts
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 216674 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepET3c
- Backbone size w/o insert (bp) 4604
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameterminal deoxynucleotidyl transferase
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Alt nameTdT
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SpeciesBos taurus
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Insert Size (bp)1152
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Mutation1-138 truncation
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GenBank IDNP_803461.1
- Promoter T7
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Tag
/ Fusion Protein
- his-tag (N terminal on insert)
Cloning Information
- Cloning method Gene Synthesis
- 5′ sequencing primer T7 promoter
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET-hisTdT was a gift from Po-Min Chiang (Addgene plasmid # 216674 ; http://n2t.net/addgene:216674 ; RRID:Addgene_216674)