pNR6
(Plasmid #21677)

• Depositing Lab: James Haber
• Publication: Rudin et al Mol Cell Biol. 1988 Sep . 8(9):3918-28.

Backbone

• Vector backbone: pRK113
• Backbone manufacturer: R. Kostriken
• Backbone size w/o insert (bp): 2700
• Vector type: integration sequence
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Ura3
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 1162
• Entrez Gene: URA3 (a.k.a. YEL021W)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Hind III (not destroyed)
• 3′ cloning site: Hind III (not destroyed)
• 5′ sequencing primer: M13-forward
• 3′ sequencing primer: M13-reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Constructed by inserting a HindIII URA3 fragment into the HindIII
site of the multiple cloning site of plasmid pRK113.

Contains the 117-bp HO cut site of
MATa inserted between the HincII and BamHI sites of pUC9 and the URA3 1.17-kb HindIII fragment at the HindlIl
site transcribed away from the HO cut site.