|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21677||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerR. Kostriken
- Backbone size w/o insert (bp) 2700
Vector typeintegration sequence
Growth in Bacteria
SpeciesS. cerevisiae (budding yeast)
Insert Size (bp)1162
Entrez GeneURA3 (a.k.a. YEL021W)
- Cloning method Restriction Enzyme
- 5′ cloning site Hind III (not destroyed)
- 3′ cloning site Hind III (not destroyed)
- 5′ sequencing primer M13-forward
- 3′ sequencing primer M13-reverse (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Constructed by inserting a HindIII URA3 fragment into the HindIII
site of the multiple cloning site of plasmid pRK113.
Contains the 117-bp HO cut site of
MATa inserted between the HincII and BamHI sites of pUC9 and the URA3 1.17-kb HindIII fragment at the HindlIl
site transcribed away from the HO cut site.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pNR6 was a gift from James Haber (Addgene plasmid # 21677 ; http://n2t.net/addgene:21677 ; RRID:Addgene_21677)
For your References section:Efficient repair of HO-induced chromosomal breaks in Saccharomyces cerevisiae by recombination between flanking homologous sequences. Rudin N, Haber JE. Mol Cell Biol. 1988 Sep . 8(9):3918-28. PubMed 3065627