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pBABE 3xHA-MKK6-EE puro
(Plasmid #216791)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216791 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pBABE puro
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MKK6
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1095
  • Mutation
    S207E and T211E, constitutively active
  • Entrez Gene
    MAP2K6 (a.k.a. MAPKK6, MEK6, MKK6, PRKMK6, SAPKK-3, SAPKK3)
  • Promoter LTR
  • Tag / Fusion Protein
    • 3x-HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site SalI (not destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid was created by PCR cloning pGEX-4T-1 3xHA-MKK6-EE (Addgene #47579) using BglII (5') and SalI (3') and inserting into pBABE puro that had been digested by BamHI (5') and SalI (3')

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBABE 3xHA-MKK6-EE puro was a gift from Kevin Janes (Addgene plasmid # 216791 ; http://n2t.net/addgene:216791 ; RRID:Addgene_216791)

  • For your References section:

    Three modes of viral adaption by the heart. Griffiths CD, Shah M, Shao W, Borgman CA, Janes KA. Sci Adv. 2024 Nov 15;10(46):eadp6303. doi: 10.1126/sciadv.adp6303. Epub 2024 Nov 13. 10.1126/sciadv.adp6303 PubMed 39536108
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