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pET30-GBF-E6C
(Plasmid #21698)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 21698 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Protein G domain B1 fused C-terminal zinc binding domain of human papillomavirus type 16 (residue 79-142)
  • Alt name
    E6
  • Alt name
    protein G
  • Species
    Human papillomavirus type 16
  • Insert Size (bp)
    378
  • Mutation
    GB1 (1-56aa) fusion tag with mutations at three positions D22N/D36R/E42K was fused to C-terminal zinc binding domain of HPV-16 E6 (residues 79-142 with mutations at four positions C80S/C97S/C111S/C140S) with a linker AAPRGS (including a thrombin cleavage site AAPR). Y79G mutation in the E6 region.
  • Entrez Gene
    E6 (a.k.a. HpV16gp1)
  • Tag / Fusion Protein
    • Protein G B1 domain (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nde I (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET30-GBF-E6C was a gift from James Baleja (Addgene plasmid # 21698 ; http://n2t.net/addgene:21698 ; RRID:Addgene_21698)
  • For your References section:

    Determinants of stability for the E6 protein of papillomavirus type 16. Liu Y, Cherry JJ, Dineen JV, Androphy EJ, Baleja JD. J Mol Biol. 2009 Mar 6. 386(4):1123-37. 10.1016/j.jmb.2009.01.018 PubMed 19244625