|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21698||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5400
Vector typeBacterial Expression
Growth in Bacteria
Gene/Insert nameProtein G domain B1 fused C-terminal zinc binding domain of human papillomavirus type 16 (residue 79-142)
Alt nameprotein G
SpeciesHuman papillomavirus type 16
Insert Size (bp)378
MutationGB1 (1-56aa) fusion tag with mutations at three positions D22N/D36R/E42K was fused to C-terminal zinc binding domain of HPV-16 E6 (residues 79-142 with mutations at four positions C80S/C97S/C111S/C140S) with a linker AAPRGS (including a thrombin cleavage site AAPR). Y79G mutation in the E6 region.
Entrez GeneE6 (a.k.a. HpV16gp1)
/ Fusion Protein
- Protein G B1 domain (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site Nde I (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pET30-GBF-E6C was a gift from James Baleja (Addgene plasmid # 21698 ; http://n2t.net/addgene:21698 ; RRID:Addgene_21698)
For your References section:Determinants of stability for the E6 protein of papillomavirus type 16. Liu Y, Cherry JJ, Dineen JV, Androphy EJ, Baleja JD. J Mol Biol. 2009 Mar 6. 386(4):1123-37. 10.1016/j.jmb.2009.01.018 PubMed 19244625
Map uploaded by the depositor.