pAAV-ITR-CMV-mCherry-1xU6-LeuIGI1
(Plasmid #217367)

• Purpose: Expresses E. coli leucine tRNA variant "LeuIGI1" and a wild-type mCherry reporter; can be packaged into AAV
• Depositing Lab: Abhishek Chatterjee
• Publication: Huang et al ACS Synth Biol. 2024 Jul 19;13(7):2141-2149. doi: 10.1021/acssynbio.4c00196. Epub 2024 Jun 21.

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Backbone

• Vector backbone: pAAV-ITR-mCherry
• Backbone size w/o insert (bp): 5498
• Total vector size (bp): 5854
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: E. coli leucine tRNA mutant, "LeuIGI1" for TAG suppression
• Alt name: LeuIGI1
• Alt name: EcLtR
• Species: E. coli
• Insert Size (bp): 356
• Mutation: G6U, C78G, U83C
• Promoter: U6

Cloning Information

• Cloning method: Golden Gate
• 5′ sequencing primer: CTGCGGCCGCACGCGTCTCGCGGTCCAG
• 3′ sequencing primer: GCTGCTCTCGTCGATCCGCTAGC

Resource Information

• Supplemental Documents:
  • paav-itr-cmv-mcherry-1xu6-leuigi1.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry