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E. coli DNA Polymerase III subunit Delta' (holB) in pet28PP
(Plasmid #217410)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 217410 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pet28PP
  • Backbone size w/o insert (bp) 5181
  • Total vector size (bp) 6335
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    DNA polymerase III subunit Delta'
  • Alt name
    holB
  • Species
    E. coli
  • Insert Size (bp)
    1152
  • Entrez Gene
    holB (a.k.a. b1099, ECK1085)
  • Tag / Fusion Protein
    • His (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    E. coli DNA Polymerase III subunit Delta' (holB) in pet28PP was a gift from Brian Kelch (Addgene plasmid # 217410 ; http://n2t.net/addgene:217410 ; RRID:Addgene_217410)

  • For your References section:

    Differences between bacteria and eukaryotes in clamp loader mechanism, a conserved process underlying DNA replication. Landeck JT, Pajak J, Norman EK, Sedivy EL, Kelch BA. J Biol Chem. 2024 Apr;300(4):107166. doi: 10.1016/j.jbc.2024.107166. Epub 2024 Mar 14. 10.1016/j.jbc.2024.107166 PubMed 38490435
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