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Addgene

pDEST-mScarlet-ORF
(Plasmid #217454)

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Item Catalog # Description Quantity Price (USD)
Plasmid 217454 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pDEST-V2-ORF
  • Backbone manufacturer
    https://www.addgene.org/73636/
  • Backbone size (bp) 7371
  • Modifications to backbone
    Replacement of Venus fragment 2 (V2) N-terminal tag with the mScarlet fluorescent protein
  • Vector type
    Mammalian Expression ; Gateway destination vector
  • Promoter CMV
  • Selectable markers
    Zeocin
  • Tag / Fusion Protein
    • mScarlet (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEST-mScarlet-ORF was a gift from Johanna Ivaska (Addgene plasmid # 217454 ; http://n2t.net/addgene:217454 ; RRID:Addgene_217454)

  • For your References section:

    Dynamic regulation of integrin beta1 phosphorylation supports invasion of breast cancer cells. Conway JRW, Joshi O, Kaivola J, Follain G, Gounis M, Kuhl D, Ivaska J. Nat Cell Biol. 2025 May 26. doi: 10.1038/s41556-025-01663-4. 10.1038/s41556-025-01663-4 PubMed 40419795
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