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Addgene

pSEVAb837
(Plasmid #217501)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 217501 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSEVAb83
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Apramycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    superfolder green fluorescent protein (sfGFP)
  • Species
    Aequorea victoria
  • Mutation
    S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F, M153T, V163A, I171V, and A206V

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSEVAb837 was a gift from Nico Claassens (Addgene plasmid # 217501 ; http://n2t.net/addgene:217501 ; RRID:Addgene_217501)

  • For your References section:

    MOSAIC: A Highly Efficient, One-Step Recombineering Approach to Plasmid Editing and Diversification. van den Brink M, Althuis TY, Danelon C, Claassens NJ. ACS Synth Biol. 2025 Jun 5. doi: 10.1021/acssynbio.4c00657. 10.1021/acssynbio.4c00657 PubMed 40471122
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