MZB035
(Plasmid #217834)

• Purpose: Bacterial expression of human AP3D1 (1-617) and AP3S1 AP-3 core hemicomplex
• Depositing Lab: Richard Baker
• Publication: Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;121(52):e2411974121. doi: 10.1073/pnas.2411974121. Epub 2024 Dec 20.

Backbone

• Vector backbone: pETDuet-1
• Backbone manufacturer: Novagen
• Total vector size (bp): 8538
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: AP3D1
• Alt name: AP-3 complex subunit delta-1
• Species: H. sapiens (human)
• Mutation: Removed residues 618-1153 from AP3D1
• Entrez Gene: AP3D1 (a.k.a. ADTD, HPS10, hBLVR)
• Tag / Fusion Protein:
  • GST (C terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Other
• 5′ sequencing primer: T7 Promoter
• 3′ sequencing primer: pETDuet Down 1

Gene/Insert 2

• Gene/Insert name: AP3S1
• Alt name: AP-3 complex subunit sigma-1
• Species: H. sapiens (human)
• Entrez Gene: AP3S1 (a.k.a. CLAPS3, Sigma3A)

Cloning Information for Gene/Insert 2

• Cloning method: Other
• 5′ sequencing primer: pETDuet Up 2
• 3′ sequencing primer: T7 Terminator

Resource Information

• Supplemental Documents:
  • MZB035.gb
• A portion of this plasmid was derived from a plasmid made by: BioBasic

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry