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Addgene

MZB127
(Plasmid #217836)

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This material is available to academics and nonprofits only. Currently unavailable outside the U.S.
Item Catalog # Description Quantity Price (USD)
Plasmid 217836 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-21b (+)
  • Backbone manufacturer
    Novagen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Arf1, rtxA
  • Alt name
    ADP-ribosylation factor 1
  • Alt name
    MARTX, Multifunctional-autoprocessing repeats-in-toxin
  • Species
    H. sapiens (human); Vibrio cholerae
  • Mutation
    Q71L
  • Entrez Gene
    ARF1 (a.k.a. PVNH8)
  • Tags / Fusion Proteins
    • CPD (C terminal on insert)
    • 10x His (C terminal on insert)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Integrated DNA Technologies (IDT) and BioBasic

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This vector encodes for a portion of the Vibrio cholerae MARTX (rtxA) toxin. A majority of the pathogenic portions of the toxin are not present, therefore regular caution should be exercised when expressing the fusion protein.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MZB127 was a gift from Richard Baker (Addgene plasmid # 217836 ; http://n2t.net/addgene:217836 ; RRID:Addgene_217836)

  • For your References section:

    A structure-based mechanism for initiation of AP-3 coated vesicle formation. Begley M, Aragon M, Baker RW. Proc Natl Acad Sci U S A. 2024 Dec 24;121(52):e2411974121. doi: 10.1073/pnas.2411974121. Epub 2024 Dec 20. 10.1073/pnas.2411974121 PubMed 39705307
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