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Addgene

MZB139
(Plasmid #217838)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 217838 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-29b(+)
  • Backbone manufacturer
    Novagen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    SMT3
  • Alt name
    SUMO
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    SMT3 (a.k.a. YDR510W)
  • Tags / Fusion Proteins
    • 10x His (N terminal on insert)
    • SUMO (SMT3)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7 Promoter
  • 3′ sequencing primer T7 Terminator
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Integrated DNA Technologies (IDT)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MZB139 was a gift from Richard Baker (Addgene plasmid # 217838 ; http://n2t.net/addgene:217838 ; RRID:Addgene_217838)

  • For your References section:

    A structure-based mechanism for initiation of AP-3 coated vesicle formation. Begley M, Aragon M, Baker RW. Proc Natl Acad Sci U S A. 2024 Dec 24;121(52):e2411974121. doi: 10.1073/pnas.2411974121. Epub 2024 Dec 20. 10.1073/pnas.2411974121 PubMed 39705307
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