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Addgene

pBN201
(Plasmid #217917)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 217917 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBAD24
  • Backbone size w/o insert (bp) 4542
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    E. coli E69 (O9a:K30) Wzc_K540M with Y715E, Y717E, Y718E mutations
  • Insert Size (bp)
    2184
  • Mutation
    K540M, Y715E, Y717E, Y718E
  • Tag / Fusion Protein
    • hexa-histidine tag (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBN201 was a gift from James Naismith (Addgene plasmid # 217917 ; http://n2t.net/addgene:217917 ; RRID:Addgene_217917)

  • For your References section:

    Molecular basis for the phosphorylation of bacterial tyrosine kinase Wzc. Yang Y, Batista M, Clarke BR, Agyare-Tabbi MR, Song H, Kuehfuss NM, Le Bas A, Robinson CV, Whitfield C, Stansfeld PJ, Naismith JH, Liu J. Nat Commun. 2025 Apr 11;16(1):3437. doi: 10.1038/s41467-025-58693-7. 10.1038/s41467-025-58693-7 PubMed 40210632
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