PERK 2: PERK.K618A.9E10.pCDNA
(Plasmid #21815)

• Depositing Lab: David Ron
• Depositing Organization: NYU Grossman School of Medicine
  Located in United States of America
• Publication: Harding et al Nature. 1999 Jan 21. 397(6716):271-4.
• Citations: 7 Articles

Backbone

• Vector backbone: pCDNA
• Backbone size w/o insert (bp): 4756
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Stbl2
• Growth instructions: We have found that the PERK cDNA confers instability (low replication ability) to plasmids. The plasmid will be provided in the STBL2 bacterial strain (gibco) which helps alleviate this problem. To recover the plasmid, stick a sterile toothpick into the stab and streak on an LB amp plate and grow at 30C. Pick an individual colony and grow in LB amp at 30C for the highest yield of plasmid.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: PERK (K618A)
• Alt name: Eif2ak3 eukaryotic translation initiation factor 2 alpha kinase 3
• Species: M. musculus (mouse)
• Insert Size (bp): 3577
• Mutation: kinase dead K618A mutant
• Entrez Gene: Eif2ak3 (a.k.a. Pek, Perk)
• Tag / Fusion Protein:
  • Myc (9E10) (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Mouse PERK, K618A, 9E10-tagged at C-terminus, mammalian expression vector pCDNAamp.

The K->A mutation introduces a unique Nru1 site at position 5070. An EcoR1-Nru1 digest thus readily distinguishes the wild-type and mutant plasmids

DNA (Catalog # 21815-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 21815-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.