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PERK 7: PERK.K618A.9E10.pBABE
(Plasmid #21820)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 21820 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBABE-puro
  • Backbone manufacturer
    Plasmid available at Addgene (#1764)
  • Backbone size w/o insert (bp) 5150
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl2
  • Growth instructions
    We have found that the PERK cDNA confers instability (low replication ability) to plasmids. The plasmid will be provided in the STBL2 bacterial strain (gibco) which helps alleviate this problem. To recover the plasmid, stick a sterile toothpick into the stab and streak on an LB amp plate and grow at 30C. Pick an individual colony and grow in LB amp at 30C for the highest yield of plasmid.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PERK K618A
  • Alt name
    Eif2ak3 eukaryotic translation initiation factor 2 alpha kinase 3
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3500
  • Mutation
    kinase dead K618A mutant
  • Entrez Gene
    Eif2ak3 (a.k.a. PE, Pek, Perk)
  • Tag / Fusion Protein
    • Myc (9E10) (C terminal on insert)

Cloning Information

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

9E10-tagged mouse PERK K618A mutant in the pro-retroviral vector pBABEpuro

Mammalian expression plasmid encoding kinase dead K618A mutant mouse PERK fused to 9E10 epitope at C-term.

Note: Addgene has yet to be able to sequence the C-terminal Myc tag, therefore we cannot guarantee that is present.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PERK 7: PERK.K618A.9E10.pBABE was a gift from David Ron (Addgene plasmid # 21820 ; http://n2t.net/addgene:21820 ; RRID:Addgene_21820)