PERK 7: PERK.K618A.9E10.pBABE
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21820||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerPlasmid available at Addgene (#1764)
- Backbone size w/o insert (bp) 5150
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Growth instructionsWe have found that the PERK cDNA confers instability (low replication ability) to plasmids. The plasmid will be provided in the STBL2 bacterial strain (gibco) which helps alleviate this problem. To recover the plasmid, stick a sterile toothpick into the stab and streak on an LB amp plate and grow at 30C. Pick an individual colony and grow in LB amp at 30C for the highest yield of plasmid.
Gene/Insert namePERK K618A
Alt nameEif2ak3 eukaryotic translation initiation factor 2 alpha kinase 3
SpeciesM. musculus (mouse)
Insert Size (bp)3500
Mutationkinase dead K618A mutant
Entrez GeneEif2ak3 (a.k.a. PE, Pek, Perk)
/ Fusion Protein
- Myc (9E10) (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ sequencing primer pBABE 5'
- 3′ sequencing primer pBABE 3' (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
9E10-tagged mouse PERK K618A mutant in the pro-retroviral vector pBABEpuro
Mammalian expression plasmid encoding kinase dead K618A mutant mouse PERK fused to 9E10 epitope at C-term.
Note: Addgene has yet to be able to sequence the C-terminal Myc tag, therefore we cannot guarantee that is present.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:PERK 7: PERK.K618A.9E10.pBABE was a gift from David Ron (Addgene plasmid # 21820 ; http://n2t.net/addgene:21820 ; RRID:Addgene_21820)