AdeC-pET21b
(Plasmid #218482)

• Purpose: Expresses adenine deaminase in E. coli with a C-terminal his tag
• Depositing Lab: Audrey Lamb
• Publication: Pasin et al Methods Enzymol. 2024;702:51-74. doi: 10.1016/bs.mie.2024.06.013. Epub 2024 Jul 8.

Backbone

• Vector backbone: pET21b
• Backbone size w/o insert (bp): 5442
• Total vector size (bp): 7131
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Adenine deaminase
• Alt name: AdeC
• Species: Escherichia coli
• Insert Size (bp): 1788
• GenBank ID: U00096
• Tag / Fusion Protein:
  • His tag (C terminal on backbone)

Cloning Information

• Cloning method: Gene Synthesis
• 5′ sequencing primer: ATG CGT CCG GCG TAG A
• 3′ sequencing primer: TAG TTA TTG CTC AGC GGT GG

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Gene was codon optimized, synthesized and cloned into pET21b by Genscript

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry