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Bm iPGM-HiBiT
(Plasmid #218483)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 218483 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21a (+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5543
  • Total vector size (bp) 7029
  • Modifications to backbone
    codon optimized for E. coli expression
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Bm-iPGM-C-HiBIT-Thrombin-6XHis
  • Alt name
    B. malayi iPGM-C-HiBiT
  • Alt name
    ipgm-1
  • Alt name
    Bma-ipgm-1 2,3-bisphosphoglycerate-independent phosphoglycerate mutase
  • Species
    Brugia malayi
  • Insert Size (bp)
    1647
  • GenBank ID
    AAQ97626.1 6095866, XP_042930917.1
  • Promoter T7
  • Tags / Fusion Proteins
    • HiBiT Tag (C terminal on insert)
    • His-tag with thrombin site (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (unknown if destroyed)
  • 3′ cloning site BamH1 (unknown if destroyed)
  • 5′ sequencing primer T7 promotor primer #69348-3
  • 3′ sequencing primer T7 terminator primer #69337-3
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    HiBIT Tag plus Thrombin site plus AAG synthesized by Genscript and subcloned into Bm-iPGM 6xHis pET21a+ at BamH1 site

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Bm iPGM-HiBiT was a gift from James Inglese (Addgene plasmid # 218483 ; http://n2t.net/addgene:218483 ; RRID:Addgene_218483)

  • For your References section:

    A general assay platform to study protein pharmacology using ligand-dependent structural dynamics. Ciulla DA, Dranchak PK, Aitha M, van Neer RHP, Shah D, Tharakan R, Wilson KM, Wang Y, Braisted JC, Inglese J. Nat Commun. 2025 May 10;16(1):4342. doi: 10.1038/s41467-025-59658-6. 10.1038/s41467-025-59658-6 PubMed 40346061
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