peGFP C3-SPG20 8xV
(Plasmid
#218579)
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PurposeExpresses GFP-tagged human SPG20 bulky hydrophobic amino acid mutant in mammalian cells
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Depositing Lab
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Depositing OrganizationUniversitaet Duisburg-Essen
Located in Germany -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 218579 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 218579-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 218579-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepeGFP-C3
- Total vector size (bp) 6696
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSPG20
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Alt nameSpartin
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SpeciesH. sapiens (human)
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Insert Size (bp)2001
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Mutationchanged Tryptophan 419, Leucine 438, Isoleucine 449, Leucine 456, Phenylalanine 549, Leucine 556, Isoleucine 563 and Isoleucine 609 to Valine
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Entrez GeneSPART (a.k.a. SPG20, TAHCCP1)
- Promoter CMV
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Tag
/ Fusion Protein
- eGFP (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMVfor
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 218579-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 218579-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
peGFP C3-SPG20 8xV was a gift from Hemmo Meyer (Addgene plasmid # 218579 ; http://n2t.net/addgene:218579 ; RRID:Addgene_218579) -
For your References section:
Lysosomal damage sensing and lysophagy initiation by SPG20-ITCH. Gahlot P, Kravic B, Rota G, van den Boom J, Levantovsky S, Schulze N, Maspero E, Polo S, Behrends C, Meyer H. Mol Cell. 2024 Mar 14:S1097-2765(24)00172-2. doi: 10.1016/j.molcel.2024.02.029. 10.1016/j.molcel.2024.02.029 PubMed 38503285
