peGFP C2-IST1
(Plasmid #218582)

• Purpose: Expresses GFP-tagged human IST1 in mammalian cells
• Depositing Lab: Hemmo Meyer
• Publication: Gahlot et al Mol Cell. 2024 Mar 14:S1097-2765(24)00172-2. doi: 10.1016/j.molcel.2024.02.029.

Backbone

• Vector backbone: peGFP-C2
• Total vector size (bp): 5826
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: IST1
• Species: H. sapiens (human)
• Insert Size (bp): 1101
• Entrez Gene: IST1 (a.k.a. CHMP8, OLC1)
• Promoter: CMV
• Tag / Fusion Protein:
  • eGFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: peGFP-for
• 3′ sequencing primer: peGFP-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Pinki Gahlot, Bojana Kravic, Giulia Rota, Johannes van den Boom, Sophie Levantovsky, Nina Schulze, Elena Maspero, Simona Polo, Christian Behrends, Hemmo Meyer,
Lysosomal damage sensing and lysophagy initiation by SPG20-ITCH,
Molecular Cell,
2024