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M3-dCAS9-dDNMT3A (dCas9-d3A)
(Plasmid #218787)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 218787 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pdCas9-DNMT3A-EGFP
  • Total vector size (bp) 11740
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    M3-dCAS9-dDNMT3A (dCas9-d3A)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    6279

Gene/Insert 2

  • Gene/Insert name
    GFP-T2A-Puro
  • Species
    H. sapiens (human)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.03.15.641804 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    M3-dCAS9-dDNMT3A (dCas9-d3A) was a gift from Maja Jagodic (Addgene plasmid # 218787 ; http://n2t.net/addgene:218787 ; RRID:Addgene_218787)

  • For your References section:

    Systematic comparison of dCas9-based DNA methylation epimodifiers over time indicates efficient on-target and widespread off-target effects. Kakhki MP, Rangani F, Ewing E, Cucuzza CS, Zheleznyakova G, Kalomoiri M, Badam TVS, Covacu R, Andreou I, Needhamsen M, Kular L, Jagodic M. bioRxiv 2025.03.15.641804; doi: 10.1101/2025.03.15.641804 10.1101/2025.03.15.641804
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