pTrcHisA-NLS-BFP-mEosEM
(Plasmid
#218934)
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PurposeExpress the nuclear import substrate NLS-BFP-mEosEM.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 218934 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTrcHisA
- Backbone size w/o insert (bp) 4414
- Total vector size (bp) 5753
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameBFP-mEosEM fusion protein
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SpeciesAequorea victori, Lobophyllia hemprichii
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Insert Size (bp)1482
- Promoter T7
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Tags
/ Fusion Proteins
- SV40 nuclear localization sequence (NLS) (N terminal on insert)
- 6xHis (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer trc promotor (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byThe template plasmid was pNLS-2xBFP (Addgene #176151), which was first reported in Chowdhury, et al. (2022) Nat. Cell Biol. 24:112-122. The mEosEM gene was a gift from P. Xu (Addgene ID#132708), which was first reported in Fu, et al. (2020) Nat. Meth. 17:55-58.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTrcHisA-NLS-BFP-mEosEM was a gift from Siegfried Musser (Addgene plasmid # 218934 ; http://n2t.net/addgene:218934 ; RRID:Addgene_218934) -
For your References section:
Overlapping nuclear import and export paths unveiled by two-colour MINFLUX. Sau A, Schnorrenberg S, Huang Z, Bandyopadhyay D, Sharma A, Gurth CM, Dave S, Musser SM. Nature. 2025 Mar 19. doi: 10.1038/s41586-025-08738-0. 10.1038/s41586-025-08738-0 PubMed 40108461