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SFFV-PPARG-Brd 155
(Plasmid #219119)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 219119 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    SFFV-MCS
  • Backbone size w/o insert (bp) 6638
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PPARG
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1434
  • Entrez Gene
    PPARG (a.k.a. CIMT1, GLM1, NR1C3, PPARG1, PPARG2, PPARG5, PPARgamma)
  • Promoter The spleen focus-forming virus (SFFV) promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bsp119I (not destroyed)
  • 3′ cloning site BstHI (not destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Barcode sequence cloned into XbaI restriction site. Design of plasmid is as follows: SFFV-[TF sequence]-WPRE-[Barcode sequence].

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SFFV-PPARG-Brd 155 was a gift from Filipe Pereira (Addgene plasmid # 219119 ; http://n2t.net/addgene:219119 ; RRID:Addgene_219119)

  • For your References section:

    A combinatorial transcription factor screening platform for immune cell reprogramming. Kurochkin I, Altman AR, Caiado I, Pértiga-Cabral D, Halitzki E, Minaeva M, Zimmermannová O, Henriques-Oliveira L, Klein D, Nair M, Oliveira D, Rabanal Cajal L, Knittel R, Feick C, Ringnér M, Martin M, Cirovic B, Pires CF, Rosa FF, Sitnicka E, Theis FJ, Pereira C-F. Cell Syst. 2026 Jan 14, https://doi.org/10.1016/j.cels.2025.101457 10.1016/j.cels.2025.101457
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