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MYC-hTRAK1(S200A, S201A, S719A, T834A, S919A)
(Plasmid #219574)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 219574 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pCMV-Tag3A-myc vector
  • Backbone manufacturer
    Agilent
  • Backbone size w/o insert (bp) 4333
  • Total vector size (bp) 7198
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    trak1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2910
  • Mutation
    S200A, S201A, S719A, T834A, S919A mutations
  • Entrez Gene
    TRAK1 (a.k.a. DEE68, EIEE68, MILT1, OIP106)
  • Promoter CMV
  • Tag / Fusion Protein
    • MYC (N terminal on insert)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    This plasmid was made by performing site directed mutagenesis of Addgene plasmid 225204

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MYC-hTRAK1(S200A, S201A, S719A, T834A, S919A) was a gift from Thomas Schwarz (Addgene plasmid # 219574 ; http://n2t.net/addgene:219574 ; RRID:Addgene_219574)

  • For your References section:

    Energy stress activates AMPK to arrest mitochondria via phosphorylation of TRAK1. Falk JE, Henke T, Gowrisankaran S, Wanderoy S, Basu H, Greally S, Steen J, Schwarz TL. J Cell Biol. 2026 Apr 6;225(4):e202501023. doi: 10.1083/jcb.202501023. Epub 2026 Jan 30. 10.1083/jcb.202501023 PubMed 41615403
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