apt-CBE-SL3
              
              
                (Plasmid
                
                #219940)
              
            
            
            
          - 
            PurposeMammalian expression of apt-CBE-SL3 protein (MCP_16aa-linker_evoAPOBEC1) with P2A-EGFP marker
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              Depositing Lab
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          Sequence Information
 
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 219940 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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            Vector backbonepCMV
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              Vector typeMammalian Expression, CRISPR
 
Growth in Bacteria
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            Bacterial Resistance(s)Ampicillin, 100 μg/mL
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            Growth Temperature37°C
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            Growth Strain(s)DH5alpha
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            Copy numberHigh Copy
 
Gene/Insert
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                Gene/Insert nameMCP_16-aa_evo-rA1_2xUGI
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                  Alt nameapt-CBE-SL3 (protein component)
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                    SpeciesH. sapiens (human), Synthetic
 - Promoter CMV
 
Cloning Information
- Cloning method Other
 
Resource Information
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            Supplemental Documents
 
Terms and Licenses
- 
        Academic/Nonprofit Terms
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      Industry Terms
- Not Available to Industry
 
 
Trademarks:
- Zeocin® is an InvivoGen trademark.
 
Depositor Comments
QTC294
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
apt-CBE-SL3 was a gift from Alexis Komor (Addgene plasmid # 219940 ; http://n2t.net/addgene:219940 ; RRID:Addgene_219940) - 
                
For your References section:
Development of multiplexed orthogonal base editor (MOBE) systems. Cowan QT, Gu S, Gu W, Ranzau BL, Simonson TS, Komor AC. Nat Biotechnol. 2024 May 21. doi: 10.1038/s41587-024-02240-0. 10.1038/s41587-024-02240-0 PubMed 38773305