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hKCNQ1 pCDNA3.1(-)
(Plasmid #219953)

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Item Catalog # Description Quantity Price (USD)
Plasmid 219953 Standard format: Plasmid sent in bacteria as agar stab 1 $89 *
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Backbone

  • Vector backbone
    pcDNA3.1(-)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5386
  • Total vector size (bp) 7445
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KCNQ1
  • Alt name
    Kv7.1
  • Alt name
    KvLQT1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2059
  • Mutation
    contains silent sequence variations
  • GenBank ID
    NM_000218.3
  • Entrez Gene
    KCNQ1 (a.k.a. ATFB1, ATFB3, JLNS1, KCNA8, KCNA9, KVLQT1, Kv1.9, Kv7.1, LQT, LQT1, RWS, SQT2, WRS)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer TAATACGACTCACTATAGGG
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hKCNQ1 pCDNA3.1(-) was a gift from Niels Decher (Addgene plasmid # 219953 ; http://n2t.net/addgene:219953 ; RRID:Addgene_219953)

  • For your References section:

    KCNQ1 is an essential mediator of the sex-dependent perception of moderate cold temperatures. Kiper AK, Wegner S, Kadala A, Rinne S, Schutte S, Winter Z, Bertoune MAR, Touska F, Matschke V, Wrobel E, Streit AK, Lang F, Schmidt C, Schulze-Bahr E, Schafer MK, Voelkl J, Seebohm G, Zimmermann K, Decher N. Proc Natl Acad Sci U S A. 2024 Jun 18;121(25):e2322475121. doi: 10.1073/pnas.2322475121. Epub 2024 Jun 10. 10.1073/pnas.2322475121 PubMed 38857404
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