pDEST17-NUDT5
(Plasmid
#219955)
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PurposeFor protein purification from Ecoli
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 219955 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepDEST17
- Total vector size (bp) 5359
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNUDT5
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SpeciesH. sapiens (human)
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Insert Size (bp)654
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Entrez GeneNUDT5 (a.k.a. YSA1, YSA1H, YSAH1, hNUDT5)
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Tag
/ Fusion Protein
- 6xHis (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer T7F
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.21203/rs.3.rs-4644671/v1 for ResearchSquare preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDEST17-NUDT5 was a gift from Karla Feijs (Addgene plasmid # 219955 ; http://n2t.net/addgene:219955 ; RRID:Addgene_219955) -
For your References section:
Family-wide analysis of human macrodomains reveals novel activities and identifies PARG as most efficient ADPr-RNA hydrolase. Weixler L, Zaja R, Ikenga NJ, Siefert J, Mohan G, Aydin G, Wijngaarden S, Filippov DV, Luscher B, Feijs-Zaja KLH. Commun Biol. 2025 Mar 18;8(1):453. doi: 10.1038/s42003-025-07901-7. 10.1038/s42003-025-07901-7 PubMed 40102620