pSBLego
(Plasmid
#219967)
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Purpose(Empty Backbone) The pSBLego vector enables sybodies for the Legobody method with a PelB signal for periplasmic expression and a "SLEHHHHHH" motif for purification and Legobody Fab binding.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 219967 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSBLego
- Backbone size (bp) 5740
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Vector typeBacterial Expression
- Promoter pBAD
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Tags
/ Fusion Proteins
- pelB signal sequence (N terminal on backbone)
- SLEHHHHHH (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Cloning Information
- Cloning method Golden Gate
- 5′ sequencing primer pBAD-for
- 3′ sequencing primer pBAD-rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSBLego was a gift from Markus Seeger (Addgene plasmid # 219967 ; http://n2t.net/addgene:219967 ; RRID:Addgene_219967) -
For your References section:
Rigid enlargement of sybodies with antibody fragments for cryo-EM analyses of small membrane proteins. Ackle F, Thavarasah S, Earp JC, Seeger MA. Sci Rep. 2025 Mar 19;15(1):9460. doi: 10.1038/s41598-025-92950-5. 10.1038/s41598-025-92950-5 PubMed 40108246
