pNES-EGFP-C1-flex-PABD-Spo20
(Plasmid
#220081)
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PurposeSpo20-PABD PA biosensor with a flexible linker to aid in nuclear export
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 220081 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepEGFP-C1
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSpo20
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)123
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Mutationamino acids 51-91
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Entrez GeneSPO20 (a.k.a. YMR017W, DBI9)
- Promoter CMV
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Tag
/ Fusion Protein
- X. leavis map2k1.L(32-44)-EGFP-SGGGSGGS (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNES-EGFP-C1-flex-PABD-Spo20 was a gift from Gerry Hammond (Addgene plasmid # 220081 ; http://n2t.net/addgene:220081 ; RRID:Addgene_220081) -
For your References section:
PILS-Nir1 is a sensitive phosphatidic acid biosensor that reveals mechanisms of lipid production. Weckerly CC, Rahn TA, Ehrlich M, Wills RC, Pemberton JG, Airola MV, Hammond GRV. J Cell Biol. 2025 Nov 3;224(11):e202405174. doi: 10.1083/jcb.202405174. Epub 2025 Sep 9. 10.1083/jcb.202405174 PubMed 40923975